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- Title
Selective Inhibition of IgE versus β<sup>2</sup>-Microglobulin in Human U--266 Myeloma Cell Line Treated with T--Cell--Derived Factors.
- Authors
Rossi, P.; Galli, E.; Gidlund, M.; Salahuddin, Z.; Laan, K.; Wigzell, H.
- Abstract
Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the IS' fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of β2-microglobulin synthesis showed no effect of T-cells or T-cell-derived supernatants. In addition, several human T-cell lymphoma-leukaemia virus I-transformed T-cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and four out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcriptional level. The results indicate that the T-cell-myeloma system is an interesting model to define better the regulation of IgE in the human.
- Subjects
T cells; IMMUNOGLOBULIN E; LYMPHOCYTES; LYMPHOMAS; LEUKEMIA; ANEMIA
- Publication
Scandinavian Journal of Immunology, 1985, Vol 22, Issue 1, p33
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1985.tb01857.x