We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prostaglandin E[sub 2] Induces Ca[sup 2+] Release from Ryanodine/Caffeine-Sensitive Stores in Bovine Adrenal Medullary Cells via EP[sub 1]-Like Receptors.
- Authors
Shibuya, Izumi; Tanaka, Keiko; Uezono, Yasuhito; Ueta, Yoichi; Toyohira, Yumiko; Yanagihara, Nobuyuki; Izumi, Futoshi; Yamashita, Hiroshi
- Abstract
Abstract: Prostaglandin E[sub 2] (PGE[sub 2]) causes Ca[sup 2+] release from intracellular Ca[sup 2+] stores and stimulates phosphoinositide metabolism in bovine adrenal medullary cells. These results have been interpreted as PGE[sub 2] induces Ca[sup 2+] release from inositol trisphosphate (IP[sub 3])-sensitive stores. However, we have recently shown that pituitary adenylate cyclase-activating polypeptide (PACAP), bradykinin, and angiotensin II release Ca[sup 2+] from caffeine/ryanodine-sensitive stores, although they cause a concomitant increase of intracellular IP[sub 3]. In light of these results, the mechanism of PGE[sub 2]-induced Ca[sup 2+] release was investigated in the present study. PGE[sub 2] dose-dependently caused a transient but consistent Ca[sup 2+] release from internal Ca[sup 2+] stores. The PGE[sub 2]induced Ca[sup 2+] release was unaffected by cinnarizine, a blocker of IP[sub 3]-induced Ca[sup 2+] release. By contrast, it was potently inhibited by prior application of caffeine and ryanodine. Although IP[sub 3] production in response to PGE[sub 2] was abolished by the phospholipase C inhibitor U-73122, Ca[sup 2+] release in response to PGE[sub 2] was unaffected by U-73122. The PGE[sub 2]-induced Ca[sup 2+] release was unaffected by Rp-adenosine 3',5'-cyclic monophosphothioate, an inhibitor of protein kinase A, and forskolin, a cyclic AMP (cAMP)-elevating agent, did not cause Ca[sup 2+] release. The EP[sub 1] agonist 17-phenyl-trinor PGE[sub 2] and the EP[sub 1]/EP[sub 3] agonist sulprostone mimicked the Ca[sup 2+]-releasing effects of PGE[sub 2], whereas the EP[sub 2] agonist butaprost or the EP[sub 2]/EP[sub 3] agonist misoprostol caused little or no Ca[sup 2+] release. The EP[sub 1] antagonist...
- Subjects
PROSTAGLANDINS; CALCIUM; PHOSPHOINOSITIDES; CELL membranes
- Publication
Journal of Neurochemistry, 1999, Vol 73, Issue 5, p2167
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1046/j.1471-4159.1999.02167.x