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- Title
Net fluxes of ammonium and nitrate in association with H fluxes in fine roots of Populus popularis.
- Authors
Luo, Jie; Qin, Jingjing; He, Fangfang; Li, Hong; Liu, Tongxian; Polle, Andrea; Peng, Changhui; Luo, Zhi-Bin
- Abstract
Poplar plants are cultivated as woody crops, which are often fertilized by addition of ammonium (NH) and/or nitrate (NO) to improve yields. However, little is known about net NH/NO fluxes and their relation with H fluxes in poplar roots. In this study, net NH/NO fluxes in association with H fluxes were measured non-invasively using scanning ion-selective electrode technique in fine roots of Populus popularis. Spatial variability of NH and NO fluxes was found along root tips of P. popularis. The maximal net uptake of NH and NO occurred, respectively, at 10 and 15 mm from poplar root tips. Net NH uptake was induced by ca. 48 % with provision of NO together, but net NO uptake was inhibited by ca. 39 % with the presence of NH in poplar roots. Furthermore, inactivation of plasma membrane (PM) H-ATPases by orthovanadate markedly inhibited net NH/NO uptake and even led to net NH release with NO co-provision. Linear correlations were observed between net NH/NO and H fluxes in poplar roots except that no correlation was found between net NH and H fluxes in roots exposed to NHCl and 0 mM vanadate. These results indicate that root tips play a key role in NH/NO uptake and that net NH/NO fluxes and the interaction of net fluxes of both ions are tightly associated with H fluxes in poplar roots.
- Subjects
AMMONIUM; ELECTRODES; DIFFERENCES; POPLARS; CELL membranes
- Publication
Planta: An International Journal of Plant Biology, 2013, Vol 237, Issue 4, p919
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-012-1807-7