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- Title
Molecular tools for gene manipulation in filamentous fungi.
- Authors
Wang, Shunxian; Chen, Haiqin; Tang, Xin; Zhang, Hao; Chen, Wei; Chen, Yong
- Abstract
Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technology. This review describes random gene integration constructed by restriction enzyme-mediated integration (REMI); Agrobacterium-mediated transformation (AMT); transposon-arrayed gene knockout (TAGKO); gene targeting technology, mainly about homologous recombination; and modern gene editing strategies containing transcription activator-like effector nucleases (TALENs) and a clustered regularly interspaced short palindromic repeat/associated protein system (CRISPR/Cas) developed in filamentous fungi and RNA technology including RNA interference (RNAi) and ribozymes. This review describes historical and modern gene manipulation methods in filamentous fungi and presents the molecular tools available to researchers investigating filamentous fungi. The biggest difference of this review from the previous ones is the addition of successful application and details of the promising gene editing tool CRISPR/Cas9 system in filamentous fungi.
- Subjects
FILAMENTOUS fungi; FUNCTIONAL genomics; PATHOGENIC microorganisms; GENE targeting; AGROBACTERIUM
- Publication
Applied Microbiology & Biotechnology, 2017, Vol 101, Issue 22, p8063
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-017-8486-z