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- Title
Malate synthase gene AoMls in the nematode-trapping fungus Arthrobotrys oligospora contributes to conidiation, trap formation, and pathogenicity.
- Authors
Zhao, Xinying; Wang, Yunchuan; Zhao, Yong; Huang, Yan; Zhang, Ke-Qin; Yang, Jinkui
- Abstract
Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified the AoMls gene from the nematode-trapping fungus Arthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function of AoMls in A. oligospora, we disrupted it by homologous recombination, and the Δ AoMls mutants were confirmed by PCR and Southern blot analyses. The growth rate and colony morphology of the Δ AoMls mutants showed no obvious difference from the wild-type strains on potato dextrose agar (PDA) plate. However, the disruption of gene AoMls led to a significant reduction in conidiation, failure to utilize fatty acids and sodium acetate for growth, and its conidia were unable to germinate on minimal medium supplemented with sodium oleate. In addition, the trap formation was retarded in the Δ AoMls mutants, which only produced immature traps containing one or two rings. Moreover, the nematicidal activity of the Δ AoMls mutants was significantly decreased. Our results suggest that the gene AoMls plays an important role in conidiation, trap formation and pathogenicity of A. oligospora.
- Subjects
MALATE synthase; NEMATODE-destroying fungi; NEMATODES; ARTHROBOTRYS; CONIDIATION; PREDACIOUS fungi
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 6, p2555
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-5432-6