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- Title
Schistosoma japonicum: screening of cercariae cDNA library by specific single-chain antibody against SIEA26-28 ku and immunization experiment of the recombinant plasmids containing the selected genes.
- Authors
Dong-mei Gao; Shi-ping Wang; Zhuo He; Ming-chiu Fung; Ming-she Liu; Lu-xin Yu; Xiu-chun Chen
- Abstract
To obtain the gene encoding SIEA26-28 ku, which has been proven to be a potential anti-schistosomiasis vaccine candidate, screening Schistosoma japonicum ( Sj) cercariae cDNA library with soluble specific single-chain antibody (SIEA26-28 ku-scFv) was performed. A large amount of specific single-chain antibody was harvested through construction of recombinant expression vector pET32a/scFv. The protein was purified and characterized. By using this protein (PET32a-scFv) as a probe, S. japonicum cercariae cDNA library was screened. Two strong positive clones were selected, and their eukaryotic recombinant plasmids were constructed. These genes were named as S. japonicum ribosomal protein S4 ( SjRPS4) and S. japonicum ribosomal protein L7 ( SjRPL7), respectively. Experiments of mice showed that both SjRPS4 and SjRPL7 DNA vaccines could induce significant immunoprotection. Result of these experiments further proved that the specific single-chain antibody is a very valuable tool in screening of cDNA library to get the corresponding molecules.
- Subjects
SCHISTOSOMA japonicum; SCHISTOSOMIASIS; DNA vaccines; VACCINES; IMMUNOGLOBULINS; PLASMIDS
- Publication
Parasitology Research, 2010, Vol 107, Issue 1, p127
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-010-1846-6