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- Title
Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes.
- Authors
Kostin, N.; Ponomarenko, N.; Isaev, V.; Rudenskaya, G.; Bobik, T.; Gabibov, A.; Smirnov, I.
- Abstract
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
- Subjects
SERPINS; PARALITHODES; LITHODIDAE; MEDICAL research; PROTEINS
- Publication
Bulletin of Experimental Biology & Medicine, 2017, Vol 163, Issue 2, p210
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-017-3768-0