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- Title
The application of in vitro model on the evaluation of feeding preference and fermentation characteristics of koala.
- Authors
Wu, Y. J.; Liu, J. T.; Wang, Y. M.; Wang, J. B.; Lin, C. Y.; Lin, T. Y.; Li, Y. W.; Liu, H. T.; Chou, C. H.; Wang, H. T.
- Abstract
This study is based on the koala's eating scores of eucalyptus leaves and the components assay result to investigate the relation between koala's feeding preference and gut fermentation characteristics by in vitro model with simulating the digestion process. Collected leaves were freeze-dried and ground, then digested with flask method under 37°C, and digested residue was inoculated with fresh feces for in vitro fermentation. Combine in vitro fermentation products assay with long-term fecal microbiota assay to monitor gut health situation. A positive correlation was shown between the eating score and moisture of the leaves, but no significant difference of crude protein and neutral-detergent fiber levels among preferred eating leaves. Male koala feces inoculation resulted in a higher volatile fatty acid (VFA) content. No significant difference in the VFA concentration at different sampling times after fermentation, both acetic acid and lactic acid were higher in the fermentation products. However, the proportion of butyric acid in total VFA was higher than other herbivores. According to the characteristics of the eucalyptus leaves fermentation product established by the in vitro digestion model and compares the leaf composition, it could lead us to understand the effect of eating different eucalyptus leaves on the nutritional value and health situation of the koala.
- Subjects
KOALA; EUCALYPTUS; ANIMAL feeding; FERMENTATION of feeds; LACTIC acid; ACETIC acid
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p208
- ISSN
0253-9187
- Publication type
Article