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- Title
Needle-free topical electroporation improves gene expression from plasmids administered in porcine skin
- Authors
Babiuk, Shawn; Baca-Estrada, Maria E.; Foldvari, Marianna; Baizer, Lawrence; Stout, Richard; Storms, Michael; Rabussay, Dietmar; Widera, Georg; Babiuk, Lorne
- Abstract
Electroporation has been shown to increase the potency of DNA vaccines that have demonstrated significant potential in mice. However, there is a need to develop noninvasive or minimally invasive vaccination methods. In pigs, in vivo gene expression was assessed to compare intradermal needle injection to a needle-free dermal BioJect as a means of delivery of plasmids. Each administration method was further tested with and without surface electroporation. Experiments with plasmid DNA encoding luciferase demonstrated that needle-free administration results in higher gene expression levels than needle injection. Electroporation enhanced gene expression for both intradermal delivery methods. Needle-free plasmid injection in combination with electroporation led to a more rapid induction of immune responses compared to other methods of plasmid administration. It was concluded that needle-free topical electroporation significantly enhances gene expression, possibly by improving cellular uptake of plasmid DNA.
- Subjects
ELECTROPORATION; DNA vaccines; GENE expression; IMMUNE response
- Publication
Molecular Therapy, 2003, Vol 8, Issue 6, p992
- ISSN
1525-0016
- Publication type
Article
- DOI
10.1016/j.ymthe.2003.09.008