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- Title
Determinación conjunta de funcionalidad de membrana plasmática e integridad de membrana acrosomal en espermatozoides de toro: Resultados preliminares.
- Authors
Torres, P.; Cartelle, J.; Malcervelli, D.; Sassano, F.; Cisale, H.; Fischman, M. L.
- Abstract
In bulls, semen quality is decisive in the fertilization process. In addition to routine tests, it´s important to assess the functionality of the plasma membrane and the integrity of the acrosomal membrane. To evaluate the plasmatic membrane, we have the HOS test and for the acrosomal membrane, Coomassie blue staining. The aim of this study was to jointly assess the functionality of the plasma membrane using the HOS test and the integrity of the acrosomal membrane using Coomassie Blue staining. Fifty-two straws (0.25 ml) of commercial frozen bovine semen were analyzed. Smears of semen samples submitted to the HOS test were made and then stained with Coomassie Blue. As control, the conventional HOS technique was used. The results obtained (Wilcoxon test p= 0.3625; Spearman test: 0.81) indicate that there are no significant differences between the techniques. We conclude that the combination of HOS with Coomassie Blue is equivalent to the HOS technique used routinely in our laboratory. The combination of the HOS test with Coomassie Blue allows us to perform a double determination on the same smear and can be carried out in any lowcomplexity laboratory.
- Subjects
SEMEN analysis; BULLS; CELL membranes; LABORATORIES; FERTILIZATION (Biology)
- Publication
Taurus, 2023, Vol 25, Issue 98, p32
- ISSN
1515-3037
- Publication type
Article