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- Title
Production and purification of 2‐phenylethanol by Saccharomyces cerevisiae using tobacco waste extract as a substrate.
- Authors
Ye, J.; Tian, S.; Lv, L.; Ding, Y.; Xu, J.; Zhang, J.; Li, L.
- Abstract
2‐phenylethanol (2‐PE), which is extracted naturally from plant or biotechnology processing, is widely used in the food and cosmetics industries. Due to the high cost of 2‐PE production, the valorization of waste carbon to produce 2‐PE has gained increasing attention. Here, 2‐PE was produced by Saccharomyces cerevisiae using tobacco waste extract (TWE) as the substrate. Considering the toxicity of nicotine and its inhibition of 2‐PE, the tolerance of S. cerevisiae was first evaluated. The results suggested that the production of 2‐PE by S. cerevisiae in TWEs could be carried out at 2·0 mg ml−1 nicotine concentrations and may be inhibited by 1·0 mg ml−1 2‐PE. Thus, the compounds in the TWEs prepared at different temperatures were detected, and the results revealed that the TWEs prepared at 140°C contained 2·18 mg ml−1 of nicotine, had total sugar concentrations of 26·8 mg ml−1 and were suitable for 2‐PE production. Due to feedback regulation, the 2‐PE production was only 1·11 mg ml−1, and the remaining glucose concentration remained at 13·78 mg ml−1, which indicated insufficient glucose utilization. Then, in situ product recovery was further implemented to remove this inhibition; the glucose utilization (the remaining concentration decreased to 3·64 mg ml−1) increased, and the 2‐PE production increased to 1·65 mg ml−1. The 2‐PE produced in the fermentation broth was first isolated by elution from the resin with 75% ethanol and then by removing the impurities with 2·5% activated charcoal, and pure 2‐PE was identified by gas chromatography mass spectrometry. The results of this study suggest that TWE could be an alternative carbon source for 2‐PE production. This could provide an outlet tobacco waste as well as reducing the price of natural 2‐PE, although more strategies need to be explored to improve the production yield of 2‐PE by using TWE.
- Subjects
SACCHAROMYCES cerevisiae; TOBACCO use; PRODUCT recovery; IN situ processing (Mining); CHARCOAL; TOBACCO smoke; ACTIVATED carbon; NICOTINE
- Publication
Letters in Applied Microbiology, 2021, Vol 73, Issue 6, p800
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/lam.13575