We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Adaptation of the yeast Yarrowia lipolytica to heat shock.
- Authors
Biryukova, E. N.; Medentsev, A. G.; Arinbasarova, A. Yu.; Akimenko, V. K.
- Abstract
The adaptive response of the yeast Yarrowia lipolytica to heat shock has been studied. Experiments showed that, after 10 min of incubation at 45°C, the survival rate of Yarrowia lipolytica cells was less than 0.1%. Stationary-phase yeast cells were found to be more thermotolerant than exponential-phase cells. A 60-min preincubation of cells at 37°C or pretreatment with low concentrations of H2O2 (0.5 mM) or menadione (0.05 mM) made them more tolerant to heat and to oxidative stress (120 mM hydrogen peroxide). The pH dependence of yeast thermotolerance has also been studied. The adaptation of yeast cells to heat shock and oxidative stress was found to be associated with a decrease in the intracellular level of cAMP and an increase in the activity of antioxidant enzymes (catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione reductase).
- Subjects
YEAST; CELLS; OXIDATIVE stress; ENZYMES; EDIBLE fungi
- Publication
Microbiology (00262617), 2007, Vol 76, Issue 2, p158
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261707020051