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- Title
Genetic Structure and Drug Susceptibility Patterns of Mycobacterium tuberculosis Complex Strains Responsible of Human Pulmonary Tuberculosis in the Major Rearing Region in Cameroon.
- Authors
Koro Koro, Francioli; Um Boock, Alphonse; Kaiyven, Afi Leslie; Noeske, Juergen; Gutierrez, Cristina; Kuaban, Christopher; Etoa, François-Xavier; Eyangoh, Sara Irène
- Abstract
Background. Cameroon this last decade continues to present a low contribution of M. africanum and M. bovis in human tuberculosis (TB), while M. bovis was prevalent in cattle but all these pieces of information only concerned West and Center regions. Methods. We carried out the first study in Adamaoua, one of the most rearing regions of Cameroon, on the genetic structure and drug susceptibility of the MTBC strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and above. For that purpose, spoligotyping, a modified 15 standard MIRU/VNTR loci typing, and the proportion method were used. Results. Four hundred and thirty-seven MTBC isolates were analyzed by spoligotyping. Of these, 423 were identified as M. tuberculosis, within the Cameroon family being dominant with 278 (65.7%) isolates; twelve (2.75%) isolates were classified as M. africanum and two as M. bovis. MIRU/VNTR typing of the most prevalent sublineage (SIT 61) suggested that this lineage is not a unique clone as thought earlier but could constitute a group of strains implicated to different pocket of TB transmission. Only M. tuberculosis sublineages were associated with antituberculosis drug resistance. Conclusion. These results showed the weak contribution of M. africanum and M. bovis to human active pulmonary tuberculosis in Cameroon even in the rearing region.
- Subjects
CAMEROON; GENETICS of tuberculosis; DNA analysis; AMINES; CHI-squared test; FISHER exact test; GENETIC polymorphisms; GENETICS; ISONIAZID; MICROBIAL sensitivity tests; MYCOBACTERIUM; MYCOBACTERIUM tuberculosis; POPULATION geography; PROBABILITY theory; RESEARCH funding; RIFAMPIN; SPUTUM; STREPTOMYCIN; TUBERCULOSIS; CROSS-sectional method; DATA analysis software; DESCRIPTIVE statistics; SEQUENCE analysis
- Publication
BioMed Research International, 2016, Vol 2016, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2016/2904832