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- Title
Improvement of Immune and Hematopoietic Functions by Litsea japonica Fruit Extract in Cyclophosphamide-Treated BALB/c Mice.
- Authors
Ji, Seon Yeong; Bang, EunJin; Hwangbo, Hyun; Kim, Min Yeong; Kim, Da Hye; Koo, Young Tae; Kim, Jin Soo; Lee, Ki Won; Park, Sun Young; Kwon, Chan-Young; Lee, Hyesook; Kim, Gi-Young; Choi, Yung Hyun
- Abstract
Loss of immunity is an important cause in the pathology of infectious disease. This study investigates the effect of Litsea japonica fruit extract (LJFE) as a potential functional food on immunity and hematopoietic function in immunosuppressed BALB/c mice. Immunity-stimulating activity was observed in mice supplemented with LJFE at low (25 mg/kg), medium (50 mg/kg), and high (100 mg/kg) dosage for seven days after administration of cyclophosphamide. LJFE treatment significantly improved spleen injury score (p < 0.001) and body weight (p < 0.02) by approximately two-fold with a high dosage of LJFE (100 mg/kg). Spleen-derived lymphocyte analysis demonstrated that the numbers of clusters of differentiation (CD)4+ and CD8+ T-cells were notably increased by approximately two-fold (p < 0.001) with a high dosage of LJFE (100 mg/kg). In mouse splenocytes differentiated into T- and B-lymphocytes, LJFE significantly induced proliferation up to approximately 90% of control for T- (p < 0.001) and B-lymphocytes (p < 0.01) with a high dosage of LJFE (100 mg/kg). Furthermore, LJFE significantly recovered the numbers of white blood cells, red blood cells, and platelets. Enzyme-linked immunosorbent assay revealed that serum levels of immune-related cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-2, and interferon (IFN)-γ, were notably Increased. In addition, serum levels of immunoglobulin (Ig) A, IgM, and IgG were restored by LJFE treatment. This study provides a reference to use L. japonica as a functional food ingredient to improve immunity and hematological function in humans.
- Subjects
FRUIT extracts; LEUCOCYTES; ERYTHROCYTES; PATHOLOGY; ENZYME-linked immunosorbent assay; IMMUNOGLOBULIN M
- Publication
Applied Sciences (2076-3417), 2023, Vol 13, Issue 1, p145
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app13010145