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- Title
MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1.
- Authors
YUNFU LIU; XIN XU; XIANGLAI XU; SHIQI LI; ZHEN LIANG; ZHENGHUI HU; JIAN WU; YI ZHU; XIAODONG JIN; XIAO WANG; YIWEI LIN; HONG CHEN; YEQING MAO; JINDAN LUO; XIANGYI ZHENG; LIPING XIE
- Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR-93a-3p levels in prostate cancer tissues compared with that in their benign prostate hyperplasia counterparts. However, the role of miR-193a-3p has yet to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression levels of miR-193a-3p in two human prostate cancer cell lines. Forced overexpression of miR-193a-3p was established by transfecting mimics into DU-145 and PC3 cell lines. Cell proliferation and the cell cycle were assessed using a cell viability assay, flow cytometry and a colony formation assay. In addition, the target gene of miR-193a-3p was determined by a luciferase assay, RT-qPCR and western blot analysis. The regulation of the cell cycle by miR-193a-3p was also evaluated by western blotting. The results demonstrated that miR-193a-3p expression levels were lower in prostate cancer cell lines as compared with the RWPE normal prostate epithelium cell line. Subsequent gain-of-function studies revealed that stable miR-193a-3p transfection inhibited cell viability, proliferation and colony formation, and induced G1 phase arrest in prostate cancer cells. A luciferase assay and western blot analysis identified cyclin D1 (CCND1) as a direct target gene of miR-193a-3p.
- Subjects
MICRORNA; PROSTATE cancer; CANCER cell proliferation; CYCLINS; GENE expression; GENE transfection
- Publication
Oncology Letters, 2017, Vol 14, Issue 5, p5121
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2017.6865