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- Title
Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β -Annulus-dT 20 Conjugate and the Poly(A) Tail of mRNA.
- Authors
Nakamura, Yoko; Sato, Yuki; Inaba, Hiroshi; Iwasaki, Takashi; Matsuura, Kazunori
- Abstract
Messenger RNA (mRNA) drugs have attracted considerable attention as promising tools with many therapeutic applications. The efficient delivery of mRNA drugs using non-viral materials is currently being explored. We demonstrate a novel concept where mCherry mRNA bearing a poly(A) tail is encapsulated into capsids co-assembled from viral β-annulus peptides bearing a 20-mer oligothymine (dT20) at the N-terminus and unmodified peptides via hybridization of dT20 and poly(A). Dynamic light scattering measurements and transmission electron microscopy images of the mRNA-encapsulated capsids show the formation of spherical assemblies of approximately 50 nm. The encapsulated mRNA shows remarkable ribonuclease resistance. Further, modification by a cell-penetrating peptide (His16) on the capsid enables the intracellular expression of mCherry of encapsulated mRNA.
- Subjects
MESSENGER RNA; CAPSIDS; TRANSMISSION electron microscopy; TAILS
- Publication
Applied Sciences (2076-3417), 2020, Vol 10, Issue 22, p8004
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app10228004