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- Title
Diversity Analysis of an Extremely Acidic Soil in a Layer of Coal Mine Detected the Occurrence of Rare Actinobacteria.
- Authors
PIKOLI, Megga Ratnasari; SUGORO, Irawan; Suharti
- Abstract
Studies that explore the diversity of microorganisms in unusual (extreme) environments have become more common. Our research aims to predict the diversity of bacteria that inhabit an extreme environment, a coal mine's soil with pH of 2.93. Soil samples were collected from the soil at a depth of 12 meters from the surface, which is a clay layer adjacent to a coal seam in Tanjung Enim, South Sumatera, Indonesia. A culture-independent method, the polymerase chain reaction based denaturing gradient gel electrophoresis, was used to amplify the 16S rRNA gene to detect the viable-but-unculturable bacteria. Results showed that some OTUs that have never been found in the coal environment and which have phylogenetic relationships to the rare actinobacteria Actinomadura, Actinoallomurus, Actinospica, Streptacidiphilus, Aciditerrimonas, and Ferrimicrobium. Accordingly, the highly acidic soil in the coal mine is a source of rare actinobacteria that can be explored further to obtain bioactive compounds for the benefit of biotechnology.
- Subjects
INDONESIA; ACID soils; COAL mining; DENATURING gradient gel electrophoresis; ACTINOBACTERIA; MINE soils; EXTREME environments
- Publication
Walailak Journal of Science & Technology, 2020, Vol 17, Issue 6, p529
- ISSN
1686-3933
- Publication type
Article
- DOI
10.48048/wjst.2020.4380