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- Title
In vitro adherence of Candida albicans to zirconia surfaces.
- Authors
Zupancic Cepic, Lana; Dvorak, Gabriella; Piehslinger, Eva; Georgopoulos, Apostolos
- Abstract
Objectives: This study aimed to characterize surface properties such as roughness (Ra) and surface‐free energy (SFE) of glazed and polished yttria‐stabilized zirconia and to evaluate in vitro adherence of fungus Candida albicans and salivary bacteria, Staphylococcus epidermidis, mixed with C. albicans to these substrata. Additionally, the influence of salivary proteins (albumin, mucin and α‐amylase) on yeast adhesion was studied. Material and methods: Ra and SFE of glazed and polished zirconia discs were measured. Specimens were wetted with saliva and salivary proteins prior to incubation with C. albicans and mixed suspension of C. albicans and S. epidermidis for 24 hr, respectively. Microbial adhesion was quantified by counting colony‐forming units (CFU). Differences in physicochemical properties were proved by t test. "Linear mixed model" with the factors "type of surface" and "wetting media" was applied to analyse the effects on fungal adhesion (p <.05). Results: SFE and Ra of glazed specimens were significantly higher than corresponding values of polished ones. The wetting media significantly changed the fungal binding (p =.0016). Significantly higher quantities of adhering fungi were found after mucin incubation compared to saliva (p =.004). For the factor "surface" as well as the interaction between "surface" and "wetting media," no statistically significant differences have been found. In mixed suspension, the growth of Candida was completely prevented. Conclusions: Glazed and polished zirconia differs in terms of physicochemical surface properties. These differences appear to be modulated by pellicle coating affecting the biomass of adhered Candida. Mucin seems to be good binding sites for adhesion of C. albicans.
- Subjects
SALIVA microbiology; YEAST physiology; BACTERIA; BACTERIAL growth; CANDIDA albicans; CELL communication; DENTAL implants; MICROBIOLOGICAL techniques; PROTEINS; SALIVA; STAPHYLOCOCCUS; T-test (Statistics); DESCRIPTIVE statistics; IN vitro studies; SURFACE properties
- Publication
Oral Diseases, 2020, Vol 26, Issue 5, p1072
- ISSN
1354-523X
- Publication type
Article
- DOI
10.1111/odi.13319