We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Toxicity Studies of Poly(Anhydride) Nanoparticles as Carriers for Oral Drug Delivery.
- Authors
Ojer, Patricia; Cerain, Adela; Areses, Paloma; Peñuelas, Ivan; Irache, Juan
- Abstract
Purpose: To evaluate the acute and subacute toxicity of poly(anhydride) nanoparticles as carriers for oral drug/antigen delivery. Methods: Three types of poly(anhydride) nanoparticles were assayed: conventional (NP), nanoparticles containing 2-hydroxypropyl-β-cyclodextrin (NP-HPCD) and nanoparticles coated with poly(ethylene glycol) 6000 (PEG-NP). Nanoparticles were prepared by a desolvation method and characterized in terms of size, zeta potential and morphology. For in vivo oral studies, acute and sub-acute toxicity studies were performed in rats in accordance to the OECD 425 and 407 guidelines respectively. Finally, biodistribution studies were carried out after radiolabelling nanoparticles with technetium. Results: Nanoparticle formulations displayed a homogeneous size of about 180 nm and a negative zeta potential. The LD for all the nanoparticles tested was established to be higher than 2000 mg/kg bw. In the sub-chronic oral toxicity studies at two different doses (30 and 300 mg/kg bw), no evident signs of toxicity were found. Lastly, biodistribution studies demonstrated that these carriers remained in the gut with no evidences of particle translocation or distribution to other organs. Conclusions: Poly(anhydride) nanoparticles (either conventional or modified with HPCD or PEG6000) showed no toxic effects, indicating that these carriers might be a safe strategy for oral delivery of therapeutics.
- Subjects
ANHYDRIDES; NANOMEDICINE; DRUG carriers; DRUG delivery systems; DRUG toxicity; CYCLODEXTRINS; ACUTE toxicity testing
- Publication
Pharmaceutical Research, 2012, Vol 29, Issue 9, p2615
- ISSN
0724-8741
- Publication type
Article
- DOI
10.1007/s11095-012-0791-8