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- Title
Purification and some characteristics of enterocin ON-157, a bacteriocin produced by Enterococcus faecium NIAI 157.
- Authors
Ohmomo, S.; Murata, S.; Katayama, N.; Nitisinprasart, S.; Kobayashi, M.; Nakajima, T.; Yajima, M.; Nakanishi, K.
- Abstract
S. OHMOMO, S. MURATA, N. KATAYAMA, S. NITISINPRASART, M. KOBAYASHI, T. NAKAJIMA, M. YAJIMA and K. NAKANISHI.2000. Bacteriocin-like activity (BLA) was screened in 690 strains of lactic acid bacteria isolated from plant materials such as silages and fermented vegetables. Among them, a strain identified as Enterococcus faecium NIAI 157 showed a clear BLA against the indicator strain, Ent. faecium IFO 13712. The proteinaceous nature and antimicrobial activity against closely related species strongly indicated that this BLA was a bacteriocin and was designated enterocin ON-157. The bacteriocin activity of this strain was extracellularly produced in the logarithmic growth phase in MRS broth and purified by ultrafiltration, ammonium sulphate precipitation and cation-exchange chromatography. Purified enterocin ON-157 had a molecular weight of approximately 2500 Da in SDS-PAGE analysis and was easily inhibited by treatment with α-amylase and proteolytic enzymes. Enterocin ON-157 had a bactericidal mode of action and inhibited the growth of the enterococci, Lactobacillus sake and Listeria monocytogenes. Enterococcus faecium NIAI 157 harboured two plasmids, 49·0 kb and 43·7 kb, and a variant missing a larger plasmid by curing with novobiocin lost the bactriocin activity.
- Subjects
BACTERIOCINS; ENTEROCOCCUS faecalis; HYDROGEN-ion concentration
- Publication
Journal of Applied Microbiology, 2000, Vol 88, Issue 1, p81
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1046/j.1365-2672.2000.00866.x