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- Title
Mouse B- and T-Cell Colony Formation in Vitro I. Separation of Colony-Promoting and -Inhibiting Activities in Concanavalin A Rat Spleen Conditioned Medium.
- Authors
Claësson, M. H.; Nissen, M. Holst; Röpke, C.
- Abstract
Rat spleen cell cultures exposed for 24 h to concanavalin A (Con A - CM) contain, in addition to interleukin 2 (IL-2), factors that promote colony formation in vitro by mouse T cells (TCPA) and B cells (BCPA). TCPA and BCPA are separable on a Sephadex G-75 column. TCPA has a molecular weight of 15,000 daltons and shows the same elution profile as IL-2. Absorption studies with Con A-activated T cells suggested that TCPA and IL-2 are the same entity. BCPA has an apparent molecular weight of 45,000 daltons and stimulates colony formation by B lymphocytes seeded at very low cell density (104 -5 &times 104 cells/ml). In contrast to TCPA, BCPA can only be demonstrated in gel-filtered material owing to the presence of B colony suppressor activities in crude Con A-CM. Two B colony inhibitory activities were demonstrated by AcA 34 chromatography of crude Con A-CM with molecular weights of 80,000-13,000 and about 50,000, respectively. Because of the specificity, simplicity and sensitivity of B and T colony formation these assay systems should be valuable tools for in vitro testing of biological activities regulating the immune system.
- Subjects
CELL culture; MOLECULAR weights; T cells; CHROMATOGRAPHIC analysis; LEUCOCYTES; IMMUNE system
- Publication
Scandinavian Journal of Immunology, 1984, Vol 19, Issue 3, p205
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1984.tb00921.x