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- Title
DETERMINATION OF THE SPERMICIDES NONOXYNOL-9 IN A GEL PHARMACEUTICAL FORMULATION BY RPLC.
- Authors
Shabir, Ghulam A.; Bradshaw, Tony K.
- Abstract
The purpose of the research described herein was to develop and validate a reversed-phase liquid chromatographic (RPLC) method for the commercially available spermicides nonoxynol-9 (1.90-2.10%, w/w) in a gel formulation. The chromatographic separation was achieved with methanol-water (83:17, v/v) as mobile phase through a Nucleosil Cyano column by UV detection at 289 nm. The calibration curve was linear over the concentration range of 0.05-0.35 mg/mL (R2 = 0.9997). The recovery ranges were from 99.87-100.04% from a gel formulation. The mean percent relative standard deviation values for intra- and inter-day precision studies were less than 1.0%. The method was adequate sensitive with detection limit of 0.0065 μg/mL. The assay method remains unaffected by small but deliberate variations, thus it was robust.
- Subjects
SPERMICIDES; NONOXYNOL-9; CHROMATOGRAPHIC analysis; METHANOL; DISTILLED water; OLIGOMERS
- Publication
Turkish Journal of Pharmaceutical Sciences, 2011, Vol 8, Issue 1, p43
- ISSN
1304-530X
- Publication type
Article