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- Title
High P-glycoprotein-mediated export observed in patients with a history of idiopathic thrombocytopenic purpura.
- Authors
Levy, Adam S.; Cunningham-Rundles, Susanna; Mazza, BethAnne; Simm, Maciej; Gorlick, Richard; Bussel, James
- Abstract
Summary. Studies have suggested that high P-glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P-glycoprotein as the likely export protein.
- Subjects
GLYCOPROTEINS; THROMBOPENIC purpura; LYMPHOCYTES; AUTOIMMUNE diseases; PATIENTS
- Publication
British Journal of Haematology, 2002, Vol 118, Issue 3, p836
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2002.03709.x