We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of an Ene Reductase from Yeast <italic>Kluyveromyces Marxianus</italic> and Application in the Asymmetric Synthesis of (<italic>R</italic>)‐Profen Esters.
- Authors
Li, Zhining; Wang, Zexu; Meng, Ge; Lu, Hong; Huang, Zedu; Chen, Fener
- Abstract
Abstract: Given their ability to reduce activated alkenes thereby generating up to two stereogenic centers, ene reductases from the old yellow enzyme (OYE) family have received much attention as effective biocatalysts. Through genome mining, a “classical” OYE, KYE2, was identified from <italic>Kluyveromyces marxianus</italic> CBS4857 and fully characterized in vitro. This NADPH‐dependent enzyme displayed a broad substrate spectrum for the bioreduction of activated alkenes. The highly stereoselective synthesis (>95 % <italic>ee</italic>) of various (<italic>R</italic>)‐profen methyl esters, including the synthesis of (<italic>R</italic>)‐flurbiprofen methyl ester on a semi‐preparative scale further demonstrates the potential of KYE2 to be developed as a general biocatalyst for chemical synthesis.
- Subjects
KLUYVEROMYCES marxianus; ENE reactions
- Publication
Asian Journal of Organic Chemistry, 2018, Vol 7, Issue 4, p763
- ISSN
2193-5807
- Publication type
Article
- DOI
10.1002/ajoc.201800059