We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial Caco-2 cells.
- Authors
Xin Lian; Takahashi, Akira; Nakano, Masayuki; Hori, Emiko; Mawatari, Kazuaki; Harada, Nagakatsu; Hosaka, Toshio; Nakaya, Yutaka
- Abstract
Vibrio parahaemolyticus is the leading cause of gastroenteritis from seafood consumption. We tried to determine how the gene expression levels of intestinal-like epithelial cells (Caco-2 cells) and mouse intestinal loop mucosal cells change upon infection with this bacterium. Since we found the robust production of interferon alpha (IFN-α) by the V. parahaemolyticus infection, we also assessed the upregulation of a number of IFN-stimulated genes (ISGs). The expressions of IFN protein were determined by Western blotting, and the gene expressions of Caco-2 cells after V. parahaemolyticus infection were determined by quantitative real-time reverse-transcription polymerase chain reaction. Three ISGs (i.e., IFN-α-inducible protein 15, IFN-α-inducible protein 6-16, and IFN-induced protein with tetratricopeptide repeats 1) were upregulated by V. parahaemolyticus infection. Infection induced the production of IFN-α, but not IFN-β or IFN-γ. The upregulation of the 3 ISGs was suppressed by treatment with a neutralizing IFN-α antibody. Moreover, the production of infection-induced IFN-α was found in the mouse intestinal loop mucosal cells. V. parahaemolyticus infection of Caco-2 cells results in IFN-α production and the expression of IFN-regulated genes.
- Subjects
VIBRIO parahaemolyticus; POLYMERASE chain reaction; GASTROENTERITIS treatment; GENETIC regulation; GENE expression; GENETIC transcription; INTERFERONS; GLYCOPROTEINS; EPITHELIAL cells; DISEASES
- Publication
Canadian Journal of Microbiology, 2007, Vol 53, Issue 9, p1084
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W07-075