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- Title
γ-H2AX foci as indication for the DNA damage in erythrocytes of medaka (Oryzias latipes) intoxicated with 4-nonylphenol.
- Authors
Sayed, Alaa El-Din H.; Watanabe-Asaka, Tomomi; Oda, Shoji; Kashiwada, Shosaku; Mitani, Hiroshi
- Abstract
The present study aimed to investigate the genotoxicity in erythrocytes induced after exposure of medaka (Oryzias latipes) to 4-nonylphenol (4-NP). Adult female medaka fish were exposed to 4-NP at three sublethal concentrations for 15 days to compare their sensitivity with that of catfish as an aquatic model. Comet assay and γ-H2AX were used as biomarkers to detect DNA damage in erythrocytes. Exposure to 4-NP resulted in an increase in the tail moment in a dose-dependent manner. The highest level of DNA damage was recorded after exposure to 100 μg/l 4-NP. The number of foci was increased after exposure to 4-NP, indicating damage to DNA. The present results confirmed the high level of morphological alterations and apoptosis of erythrocytes detected in the first part of this study. 4-NP induced genotoxic effects in medaka, which were found to be more sensitive than catfish after exposure to 4-nonylphenol.
- Subjects
ORYZIAS latipes; DNA damage; ERYTHROCYTES; GENETIC toxicology; APOPTOSIS
- Publication
Environmental Science & Pollution Research, 2020, Vol 27, Issue 16, p18966
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-018-2985-z