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- Title
Application of membrane processes for the concentration of Symphytum officinale and Geranium robertianum extracts to obtain compounds with high anti-oxidative activity.
- Authors
PAUN, GABRIELA; NEAGU, ELENA; LITESCU, SIMONA CARMEN; ROTINBERG, PINCU; RADU, GABRIEL LUCIAN
- Abstract
The paper reports the successful application of membranes processes to obtain good quality extracts with compounds of high antioxidative activity and therapeutic value. In this study, the phenolic compounds from two plant species used in Romanian ethno-medicine were investigated and their antioxi/dant and cytotoxic activities evaluated. Three extracts prepared from Geranium robertianum and Symphytum officinale were concentrated by microfiltration and ultrafiltration. The levels of phenolic compounds and flavonoids were determined by UV-Vis spectroscopy and high-pressure liquid chromatography (HPLC). The free-radical scavenging activity of the concentrated extracts was determined by the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) method. The preliminary tests of cytotoxic activity for the concentrated extracts were performed on human epidermoid laryngeal carcinoma cell line (Hep-2p) and normal monkey kidney cells (RM). The results showed that all the concentrated extracts had a very low cytotoxicity against healthy cells, but a significant cytotoxic effect on Hep-2p tumor cells. The concentrated extracts had a high antioxidant activity (% DPPH inhibition > 80 %).
- Subjects
ARTIFICIAL membranes; COMFREY; GERANIUM robertianum; PLANT extracts; PHENOLS; ULTRAFILTRATION
- Publication
Journal of the Serbian Chemical Society, 2012, Vol 77, Issue 9, p1191
- ISSN
0352-5139
- Publication type
Article
- DOI
10.2298/JSC120106021P