We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
APEX2-based proximity proteomic analysis identifies candidate interactors for Plasmodium falciparum knob-associated histidine-rich protein in infected erythrocytes.
- Authors
Charneau, Sébastien; de Oliveira, Lucas Silva; Zenonos, Zenon; Hopp, Christine S.; Bastos, Izabela M. D.; Loew, Damarys; Lombard, Bérangère; Pandolfo Silveira, Ariane; de Carvalho Nardeli Basílio Lobo, Giovanna; Bao, Sônia Nair; Grellier, Philippe; Rayner, Julian C.
- Abstract
The interaction of Plasmodium falciparum—infected red blood cells (iRBCs) with the vascular endothelium plays a crucial role in malaria pathology and disease. KAHRP is an exported P. falciparum protein involved in iRBC remodelling, which is essential for the formation of protrusions or "knobs" on the iRBC surface. These knobs and the proteins that are concentrated within them allow the parasites to escape the immune response and host spleen clearance by mediating cytoadherence of the iRBC to the endothelial wall, but this also slows down blood circulation, leading in some cases to severe cerebral and placental complications. In this work, we have applied genetic and biochemical tools to identify proteins that interact with P. falciparum KAHRP using enhanced ascorbate peroxidase 2 (APEX2) proximity-dependent biotinylation and label-free shotgun proteomics. A total of 30 potential KAHRP-interacting candidates were identified, based on the assigned fragmented biotinylated ions. Several identified proteins have been previously reported to be part of the Maurer's clefts and knobs, where KAHRP resides. This study may contribute to a broader understanding of P. falciparum protein trafficking and knob architecture and shows for the first time the feasibility of using APEX2-proximity labelling in iRBCs.
- Subjects
PLASMODIUM falciparum; PROTEOMICS; PREGNANCY complications; CEREBRAL circulation; ERYTHROCYTES; STARTLE reaction; ERYTHROCYTE membranes
- Publication
Scientific Reports, 2024, Vol 14, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-61295-w