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- Title
Cellular Applications of DNP Solid‐State NMR – State of the Art and a Look to the Future.
- Authors
Beriashvili, David; Zhou, Jiaxin; Liu, Yangping; Folkers, Gert E.; Baldus, Marc
- Abstract
Sensitivity enhanced dynamic nuclear polarization solid‐state NMR is emerging as a powerful technique for probing the structural properties of conformationally homogenous and heterogenous biomolecular species irrespective of size at atomic resolution within their native environments. Herein we detail advancements that have made acquiring such data, specifically within the confines of intact bacterial and eukaryotic cell a reality and further discuss the type of structural information that can presently be garnered by the technique's exploitation. Subsequently, we discuss bottlenecks that have thus far curbed cellular DNP‐ssNMR's broader adoption namely due a lack of sensitivity and spectral resolution. We also explore possible solutions ranging from utilization of new pulse sequences, design of better performing polarizing agents, and application of additional biochemical/ cell biological methodologies.
- Subjects
POLARIZATION (Nuclear physics); SPECTRAL sensitivity; BACTERIAL cells; ATOMIC radius; EUKARYOTIC cells
- Publication
Chemistry - A European Journal, 2024, Vol 30, Issue 28, p1
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.202400323