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- Title
Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro.
- Authors
Sun, Li-Xin; Chen, Li-Hua; Lin, Zhi-Bin; Qin, Ying; Zhang, Jia-Qi; Yang, Jing; Ma, Jie; Ye, Ting; Li, Wei-Dong
- Abstract
Objectives Restoration of epithelial continuity in the intestinal surface after extensive destruction is important since intestinal epithelial cells stand as a boundary between the body's internal and external environment. Polysaccharides from Ganoderma lucidum (Gl -PS) may benefit intestinal epithelial wound healing in different aspects, which awaits clarification. To identify potential effects, a non-transformed small-intestinal epithelial cell line, IEC-6 cells, was used. Methods Effects on epithelial cell proliferation, migration, morphology of differentiation and transforming growth factor beta (TGF- β) protein expression, as well as the cellular ornithine decarboxylase (ODC) mRNA and c-Myc mRNA expression, were assessed, respectively, by MTT assay, wound model in vitro, observation under a microscope after hematoxylin and eosin staining, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction assays. Key findings It was shown that Gl-PS stimulated IEC-6 cell proliferation and migration significantly in a dose-dependent manner; 10 µg/ml Gl -PS improved the morphology of differentiation in IEC-6 cells. Inefficacy in expression of TGF- β in IEC-6 cells indicated a possible TGF- β independent action of Gl -PS. However, Gl -PS increased ODC mRNA and c-Myc mRNA expression in a dose-dependent manner, indicating, at least partially possible involvement of ODC and c-Myc gene expression in improvement of intestinal wound healing. Conclusions These results suggest the potential usefulness of Gl -PS to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
- Subjects
EPITHELIAL cells; POLYSACCHARIDES; POLYMERASE chain reaction; CELLS; MESSENGER RNA
- Publication
Journal of Pharmacy & Pharmacology, 2011, Vol 63, Issue 12, p1595
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/j.2042-7158.2011.01367.x