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- Title
Gene expression profiling of the response of Streptococcus pneumoniae to penicillin.
- Authors
P. David Rogers; Teresa T. Liu; Katherine S. Barker; George M. Hilliard; B. Keith English; Justin Thornton; Edwin Swiatlo; Larry S. McDaniel
- Abstract
<sec><st>Objectives</st> The aim of this study was to identify changes in the gene expression profile of <it>Streptococcus pneumoniae</it> in response to a subinhibitory concentration of penicillin in an effort to better understand mechanisms by which this organism copes with this stress. </sec> <sec><st>Methods</st> <it>S. pneumoniae</it> serotype 2 strain D39 was grown for 1 h in the presence or absence of penicillin at a concentration equivalent to half the MIC (0.03 mg/L). RNA was isolated and gene expression profiles were compared using DNA microarrays. Differential expression of select genes was confirmed by real-time RT–PCR. </sec> <sec><st>Results</st> A total of 386 genes were found to be responsive to penicillin. Up-regulated genes included those of the <it>ciaR</it>–<it>ciaH</it> operon, <it>luxS</it>, genes encoding cell envelope proteins and genes of the <it>pst</it> locus. Down-regulated genes included genes involved in competence, genes encoding capsular polysaccharide biosynthesis proteins, genes involved in fatty acid chain elongation and genes of the polyamine transporter operon. </sec> <sec><st>Conclusions</st> Altered expression of these genes reflects a protective response to perturbation of the bacterial cell wall by penicillin. Such genes may represent potential therapeutic targets for enhancing the activity of penicillin against this organism and provide insight into novel mechanisms of penicillin resistance. </sec>
- Subjects
GENES; HEREDITY; ANTIBACTERIAL agents; STREPTOCOCCUS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2007, Vol 59, Issue 4, p616
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkl560