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- Title
Effect of insertion of apoplastic invertase gene on photosynthesis of potato plants grown at various light intensities.
- Authors
Chikov, V.; Bakirova, G.; Batasheva, S.; Zamalieva, F.; Salyakhova, G.; Safiullina, G.; Sinkevich, M.; Khamidullina, L.
- Abstract
We studied the influence of yeast invertase gene ( inv), with the apoplastic localization of the enzyme, on photosynthesis of potato plants ( Solanum tuberosum L., cv. Desiree) grown at various irradiances. Plants were raised in vitro, planted in soil in gauze-insulated stands, and grown at irradiances of 100, 200, and 380 W/m of photosynthetically active radiation. Wild-type plants (WT) and the plants transformed with yeast invertase gene (B33- inv) were used. In the beginning of flowering stage, assimilation of CO and C incorporation into photosynthates were measured. Irrespective of irradiance, the carbon assimilation was higher in WT plants, than in transformed B33- inv plants. In the plants studied, we observed divergent light dependences of C inclusion into sucrose: the highest labeling was observed at low irradiance in WT plants and at high irradiance in B33- inv transformed plants. The content of C incorporated into amino acids changed in the opposite direction compared to C incorporation into sucrose. Irrespective of the plant type, similar light dependences were observed for C content in the products of glycolate metabolism and in glycerate. At the intermediate irradiance, the patterns of C distribution among photosynthetic products showed minimal differences between the plants of two types. The role of apoplast invertase in sugar export from the leaf and the possible control of plant productivity through this enzyme activity are discussed.
- Subjects
GENETIC regulation in plants; PLANT genetic transformation; ENZYME regulation; POTATOES; PHOTOSYNTHESIS genetics; PLANT assimilation; PLANT translocation; SPECTRAL irradiance
- Publication
Russian Journal of Plant Physiology, 2011, Vol 58, Issue 5, p879
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443711050049