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- Title
ASSESSMENT OF OLIVE LEAVES EXTRACT - CYTOTOXICITY IN VITRO AND ANGIOGENESIS IN OVO.
- Authors
BORUGĂ, MĂDĂLINA; ENĂTESCU, VIRGIL; PÎNZARU, IULIA; SZUHANEK, CAMELIA; MINDA, DALIANA; MARCOVICI, IASMINA; RADU, DANIELA; MARȚI, DANIELA; VLAICU, BRIGITHA; SUCIU, OANA
- Abstract
In this study, it was determined the composition of the olive leaves ethanolic extract by LC-MS, the in vitro cytotoxicity on A375 human melanoma cells was established, by means of the MTT assay (number of viable cells), the Annexin V-FITC assay (number of apoptotic cells) and in vivo irritant and toxic potential by chorioallantoic membrane assay. The extract contains a number of polyphenols, among which luteolin and rutin are the predominant compounds and triterpenic acids with a significant ursolic acid content. At the highest concentration tested, 100 μg/mL, it was noted an important cytotoxic effect on human melanoma cells, it influences the development of growing capillaries, it induces narrowed calibre vessels, but no signs of toxicity on HET-CAM were recorded, while 24 h after treatment, the extract expressed a stronger effect, upon both tumour cell growth and the vascularization of A375 melanoma cells on in vivo test.
- Subjects
OLIVE leaves; CHORIOALLANTOIS; COMPOSITION of leaves; URSOLIC acid; NEOVASCULARIZATION; HYDROXYTYROSOL
- Publication
Farmacia, 2021, Vol 69, Issue 1, p38
- ISSN
0014-8237
- Publication type
Article
- DOI
10.31925/farmacia.2021.1.5