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- Title
Characterization of Copy Number Variations in Oral Cavity Squamous Cell Carcinoma Reveals a Novel Role for MLLT3 in Cell Invasiveness.
- Authors
Wang, Chun‐I; Kao, Huang‐Kai; Chen, Ting‐Wen; Huang, Yenlin; Cheng, Hsing‐Wen; Yi, Jui‐Shan; Hung, Shao‐Yu; Wu, Chi‐Sheng; Lee, Yun‐Shien; Chang, Kai‐Ping
- Abstract
Background: DNA copy number variations (CNVs) are a hallmark of cancer, and the current study aimed to demonstrate the profile of the CNVs for oral cavity squamous cell carcinoma (OSCC) and elucidate the clinicopathological associations and molecular mechanisms of a potential marker derived from CNVs, mixed‐lineage leukemia translocated to chromosome 3 protein (MLLT3), in OSCC carcinogenesis. Materials and Methods: CNVs in 37 OSCC tissue specimens were analyzed using a high‐resolution microarray, the OncoScan array. Gene expression was analyzed by real‐time polymerase chain reaction in 127 OSCC and normal tissue samples. Cell function assays included cell cycle, migration, invasion and chromatin immunoprecipitation assays. Results: We found a novel copy number amplified region, chromosome 9p, encompassing MLLT3 via the comparison of our data set with six other OSCC genome‐wide CNV data sets. MLLT3 overexpression was associated with poorer overall survival in patients with OSCC (p =.048). MLLT3 knockdown reduced cell migration and invasion. The reduced invasion ability in MLLT3‐knockdown cells was rescued with double knockdown of MLLT3 and CBP/p300‐interacting transactivator with ED rich carboxy‐terminal domain 4 (CITED4; 21.0% vs. 61.5%). Knockdown of MLLT3 impaired disruptor of telomeric silencing‐1‐like (Dot1L)‐associated hypermethylation in the promoter of the tumor suppressor, CITED4 (p <.001), and hence dysregulated HIF‐1α‐mediated genes (TWIST, MMP1, MMP2, VIM, and CDH1) in OSCC cells. Conclusion: We identified unique CNVs in tumors of Taiwanese patients with OSCC. Notably, MLLT3 overexpression is related to the poorer prognosis of patients with OSCC and is required for Dot1L‐mediated transcriptional repression of CITED4, leading to dysregulation of HIF‐1α‐mediated genes. Implications for Practice: This article reports unique copy number variations in oral cavity squamous cell carcinoma (OSCC) tumors of Taiwanese patients. Notably, MLLT3 overexpression is related to the poorer prognosis of patients with OSCC and is required for Dot1L‐mediated transcriptional repression of CITED4, leading to dysregulation of HIF‐1α‐mediated genes. DNA copy number variations are a hallmark of cancer. This article reports a potential biomarker derived from copy number variations, MLLT3, in oral cavity squamous cell carcinoma carcinogenesis.
- Subjects
TAIWAN; DNA analysis; CANCER invasiveness; CELL cycle; CELL lines; CELL migration; DNA; GENE expression; HUMAN genome; MICROBIOLOGICAL assay; MOLECULAR biology; MOUTH tumors; HEAD &; neck cancer; POLYMERASE chain reaction; PROTEINS; SQUAMOUS cell carcinoma; TISSUE arrays; PRECIPITIN tests; IN vitro studies
- Publication
Oncologist, 2019, Vol 24, Issue 12, pe1388
- ISSN
1083-7159
- Publication type
Article
- DOI
10.1634/theoncologist.2019-0063