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- Title
甲鱼蛋蛋白水解物的体外消化及对抗氧化和d-葡萄糖背酶 抑制活性的影响.
- Authors
赵才冬; 漆姚姚; 雷楚文; 裘乐芸; 邓泽元; 郑溜丰
- Abstract
Antioxidant intervention and inhibition of a-glucosidase activity are the effective strategies for prevention and treatment of diabetes. In this study, protein of soft-shelled turtle egg was hydrolyzed by papain, and hydrolysate fraction (HF) with low molecular weight < 2.5 ku was separated by ultrafiltration. The digestive characteristic of HF and changes of antioxidative and a-glucosidase inhibitory activities were investigated using in vitro simulated gastrointestinal digestion model. The results showed that the HF had a good color (yellowish green), and its aqueous solution was colorless and transparent. The chromatographic peaks of HF were slightly altered after gastric digestion} indicating that it had a good stability in stomach. However, the original chromatographic peaks were decreased after gastrointestinal digestion, and even some peaks were disappeared. Novel chromatographic peaks were correspondingly formed} and the peptide contents were also increased, indicating that some bioactive peptides in HF were digested to produce shorter peptides. The IC values (mg/mL) of DPPH radical -scavenging activities for HF before digestion and after gastric and gastrointestinal digestions were 13.61, 6.79 and 3.56, respectively. The IC刃 values (mg/mL) of ABTS radical -scavenging activities were 18.21} 6.79 and 3,56, respectively. The ICsq values (mg/mL) of a-glucosidase inhibitory activities were 17.96, 10.81 and < 1, respectively. Therefore, HF with low molecular weight < 2,5 ku of soft-shelled turtle egg has a potential hypoglycemic 己匸 feet, and its products still has a strong or even better hypoglycemic potential after gastrointestinal digestion.
- Subjects
SOFT-shelled turtles; TURTLE eggs; PEPTIDES; MOLECULAR weights; PAPAIN; ALPHA-glucosidases; GLUCOSIDASES
- Publication
Journal of Chinese Institute of Food Science & Technology, 2022, Vol 22, Issue 10, p126
- ISSN
1009-7848
- Publication type
Article
- DOI
10.16429/j.1009-7848.2022.10.014