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- Title
Resveratrol attenuates bleomycin-induced genotoxicity, pulmonary fibrosis and DNA damage in balb/c mice with ehrlich ascites carcinoma.
- Authors
KOYUNCU, İsmail
- Abstract
Objective: Oxidative stress plays an important role in the pathogenesis and genotoxicity of bleomycin (BLM)-origin lung fibrosis. The aim of this study was to investigate the protective effect of resveratrol (RES) on lung fibrosis, DNA damage and genotoxicity developing associated with BLM in mice carrying Ehrlich ascites carcinoma. Methods: A total of 60 balb/c mice were randomly separated into 6 groups. With the exception of the Sham group, mice in the other groups were injected intraperitoneally (ip) with 3 x 105 live EAT cells, then after 24 hours, BLM only (10 mg/kg, i.p), RES only (50 mg/kg, i.p), BLM (10 mg/kg, i.p)+RES (25 mg/kg, i.p) and BLM (10 mg/kg, i.p)+RES (50 mg/kg, i.p) were applied to the mice for 6 days. With examination in serum and lung tissue of oxidant parameters such as DNA damage (comet assay and 8-OHdG), malondialdehyde (MDA), myeloperoxidase (MPO), protein carbonyl (PC), hydroxyproline (HPR), total oxidant status (TOS) and oxidative stress index (OSI) and antioxidant parameters such as superoxide dismutase (SOD), total antioxidant status (TAS) and glutathione (GSH), evaluation was made of BLM-related pulmonary toxicity and genotoxicity and the potential role of resveratrol (RES) in preventing damage. Results: The results determined that RES reduced MDA, PC, TOS, MPO OSI, HPR and DNA damage levels which showed an increase related to BLM (p<0.05) and increased GSH, TAS and SOD activities (p<0.05). It has also been found that RES inhibits BLM-induced fibrosis in histological findings. Conclusion: These results have shown that fibrosis formed as a result of lipid and protein oxidization and DNA damage caused by BLM in the lungs of EAT-carrying mice could be reduced with the application of RES. Therefore, RES could be considered as an adjuvant therapy to conventional treatment methods for pulmonary fibrosis and DNA damage of the lungs.
- Subjects
EHRLICH ascites carcinoma; RESVERATROL; BLEOMYCIN
- Publication
Bezmialem Science, 2018, Vol 6, Issue 2, p1
- ISSN
2148-2373
- Publication type
Article
- DOI
10.14235/bs.2018.2105