We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of Mac‐1 integrin in generation of extracellular vesicles with antibacterial capacity from neutrophilic granulocytes.
- Authors
Lőrincz, Ákos M.; Bartos, Balázs; Szombath, Dávid; Szeifert, Viktória; Timár, Csaba I.; Turiák, Lilla; Drahos, László; Kittel, Ágnes; Veres, Dániel S.; Kolonics, Ferenc; Mócsai, Attila; Ligeti, Erzsébet
- Abstract
Production of extracellular vesicles (EVs) involved in intercellular communication is a common capacity of most cell types. Upon encountering opsonized microorganisms, neutrophilic granulocytes release EVs that compromise bacterial growth. We carried out a systematic investigation of the involvement of potential opsonin receptors in EV‐generation from human and murine neutrophils. Applying flow cytometric, proteomic and functional analysis as well as using genetically modified mice, we demonstrate that formation of antibacterial EVs depends upon stimulation of the multifunctional Mac‐1 integrin complex, also called as complement receptor 3 (CR3), whereas activation of immunoglobulin binding Fc receptors or pattern recognition receptors alone or in combination is ineffective. Mac‐1/CR3 stimulation and downstream tyrosine kinase signalling affect both the numbers, the cargo content and the antibacterial capacity of the produced vesicles. In contrast, Mac‐1/CR3 signalling is not required for spontaneous EV formation, clearly indicating the existence of separate molecular pathways in EV biogenesis. We propose that EVs are "tailor‐made" with different composition and functional properties depending on the environmental circumstances.
- Subjects
INTEGRINS; PATTERN perception receptors; FC receptors; GRANULOCYTES; COMPLEMENT receptors; NEUTROPHILS; EXTRACELLULAR vesicles
- Publication
Journal of Extracellular Vesicles, 2020, Vol 9, Issue 1, p1
- ISSN
2001-3078
- Publication type
Article
- DOI
10.1080/20013078.2019.1698889