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- Title
Insulin and contraction increase nutritive blood flow in rat muscle in vivo determined by microdialysis ofl-[<sup>14</sup>C]glucose.
- Authors
Newman, John M. B.; Ross, Renee M.; Richards, Stephen M.; Clark, Michael G.; Rattigan, Stephen
- Abstract
In the present study, a mathematical model using the microdialysis outflow: inflow (O/I) ratio of the novel analoguel-[14C]glucose has been developed which allows the calculation of the nutritive (and non-nutritive) flow in muscle as a proportion of total blood flow. Anaesthetized rats had microdialysis probes carryingl-[14C]glucose inserted through a calf muscle group (tibialis/plantaris/gastrocnemius). The nutritive fraction of total blood flow was determined under basal conditions and in response to contraction (electrical field stimulation), insulin (hyperinsulinaemic euglycaemic clamp with 10 mU min−1 kg−1 insulin) or saline control from limb blood flow and the microdialysis O/I ratio ofl-[14C]glucose. Both contraction and insulin infusion decreased the O/I ratio ofl-[14C]glucose and increased total limb blood flow. Calculations based on mathematical models usingl-[14C]glucose O/I and limb blood flow revealed that during basal conditions, the nutritive fraction of total flow was 0.38 ± 0.06, indicating that basal flow was predominantly non-nutritive. Contraction and insulin increased the nutritive fraction to 0.82 ± 0.24 ( P < 0.05) and 0.52 ± 0.12 ( P < 0.05). Thus the increase in limb blood flow from insulin was fully accommodated by nutritive flow, while contraction increased nutritive flow at the expense of non-nutritive flow. This novel method using microdialysis and the O/I ratio ofl-[14C]glucose allows the determination of the nutritive fraction of total flow in muscle as well as the proportion of total flow that may be redistributed in response to contraction and insulin.
- Publication
Journal of Physiology, 2007, Vol 585, Issue 1, p217
- ISSN
0022-3751
- Publication type
Article
- DOI
10.1113/jphysiol.2007.138818