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- Title
IDENTIFICATION OF RESTRICTION-MODIFICATION SYSTEMS IN Clostridium butyricum USING BIOINFORMATIC TOOLS.
- Authors
ROSLAN, ROZIEFFA; PEER MOHAMED ABDUL; JAHIM, JAMALIAH M. D.
- Abstract
Genetic manipulation is an extremely robust method to enhance the yield of major fermentation product. Nevertheless, restriction-modification (RM) system is the main barrier to genetic manipulation, as it prevents foreign DNA transfer into the host. In order to investigate the presence of restriction enzymes homolog in Clostridium butyricum, a comparative analysis was performed on restriction enzyme sequences from REBASE collection against genome and amino acid sequences of C. butyricum using BLAST program. Multiple restriction enzymes homolog with more than 75% query cover were recognised in the genomes. Based on sequence analysis of five strains, Type II RM system is the most abundant (63.16%), Type IV is the second most abundant (21.05%), followed by Type III (10.53%) and Type I (5.26%). By utilising whole genome sequences and restriction enzyme database, we can comprehend the biological aspect of strain-specific RM systems to develop better strategies and techniques in order to overcome the restriction barrier in genetic manipulation.
- Subjects
CLOSTRIDIUM butyricum; NUCLEOTIDE sequencing; METHYLTRANSFERASES
- Publication
Malaysian Applied Biology, 2018, Vol 47, Issue 5, p89
- ISSN
0126-8643
- Publication type
Article