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- Title
Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner.
- Authors
Jingwei Li; Tingmin Huang; Ming Xia; Jinbiao Lu; Xiuhong Xu; Haiyi Liu; Wanping Zhang
- Abstract
Radish (Raphanus sativus L.) is an economically important vegetable worldwide, but its sustainable production and breeding are highly threatened by blight disease caused by Alternaria brassicae. Melatonin is an important growth regulator that can influence physiological activities in both plants and microbes and stimulate biotic stress resistance in plants. In this study, 0-1500 mM melatonin was exogenously applied to healthy radish seedlings, in vitro incubated A. brassicae, and diseased radish seedlings to determine the effects of melatonin on host, pathogen, and host-pathogen interaction. At sufficient concentrations (0-500 mM), melatonin enhanced growth and immunity of healthy radish seedlings by improving the function of organelles and promoting the biosynthesis of antioxidant enzymes, chitin, organic acid, and defense proteins. Interestingly, melatonin also improved colony growth, development, and virulence of A. brassicae. A strong dosage-dependent effect of melatonin was observed: 50-500 mM promoted host and pathogen vitality and resistance (500 mM was optimal) and 1500 mM inhibited these processes. Significantly less blight was observed on diseased seedlings treated with 500 mM melatonin, indicating that melatonin more strongly enhanced the growth and immunity of radish than it promoted the development and virulence of A. brassicae at this treatment concentration. These effects of MT were mediated by transcriptional changes of key genes as identified by RNA-seq, Dual RNA-seq, and qRT-PCR. The results from this work provide a theoretical basis for the application of melatonin to protect vegetable crops against pathogens.
- Subjects
RADISHES; ALTERNARIA; SUSTAINABILITY; MELATONIN; ALTERNARIA diseases; GROWTH regulators
- Publication
Frontiers in Plant Science, 2023, Vol 14, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2023.1126669