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- Title
Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis.
- Authors
Lai, P-Y; Wang, C-Y; Chen, W-Y; Kao, Y-H; Tsai, H-M; Tachibana, T; Chang, W-C; Chung, B-c
- Abstract
SF-1 (Steroidogenic Factor 1, NR5A1) is a tissue-specific transcription factor critical for the growth, development and differentiation of steroidogenic and a few other endocrine tissues. But how SF-1 regulates cell growth is not entirely clear. Here we found that SF-1 was localized to the centrosome in addition to the nucleus, and SF-1 depletion by shRNA caused centrosome over-duplication, aberrant mitosis and genomic instability, leading to a reduction of cell number. Centrosome amplification defect was rescued by both wild-type SF-1 and transcription-defective SF-1-G35E, suggesting a non-genomic activity of SF-1 involved in centrosome homeostasis. In addition, we identified in SF-1 a centrosome localization signal, whose overexpression led to reduced localization of both SF-1 and γ-tubulin to the centrosome. Our results uncover a novel role of SF-1 in the control of centrosome homeostasis and genomic stability.
- Subjects
CENTROSOMES; HOMEOSTASIS; TRANSCRIPTION factors; GROWTH factors; MITOSIS
- Publication
Cell Death & Differentiation, 2011, Vol 18, Issue 12, p1836
- ISSN
1350-9047
- Publication type
Article
- DOI
10.1038/cdd.2011.54