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- Title
Concentration and purification of polyunsaturated fatty acids from squid liver processing wastes.
- Authors
Latyshev, Nikolay A.; Ermolenko, Ekaterina V.; Kasyanov, Sergey P.
- Abstract
Eicosapentaenoic (EPA, 20:5 n-3) and docosahexaenoic (DHA, 22:6 n-3) acids were extracted from processing wastes of the liver of the squid Berryteuthis magister. The process included three steps: concentration of the total polyunsaturated fatty acids (PUFF) with urea; separation of individual FA by iodolactonization method, and obtainment of highly purified EPA and DHA by preparative HPLC. Concentration of the acids with urea was carried out at a ratio of urea/FA 3:1 at temperatures of 0 and -20°C, this allowed us to raise the concentration of EPA and DHA from7.8 and 6.5%to 32.5 and 32.0%, respectively. The iodolactonization reaction was used to obtain from the concentrate individual preparations with concentrations of 93.4% for EPA and 95.6%for DHA; the total yields of the original FA were 50.7 and 41.7%, respectively. Differences in the rates of formation of γ- and d-iodolactones made a sequential extraction possible, first of DHA from the total PUFF, then of EPA from the remaining acids. Purification of the obtained PUFF products to more than 99% purity was conducted by preparative HPLC. Practical applications: The proposed three-step procedure for separating EPA and DHA is designed to produce n-3 PUFF concentrates and highly purified EPA and DHA. The obtained products can be recommended for scientific experiments in vitro.
- Subjects
UNSATURATED fatty acids; EICOSAPENTAENOIC acid; DOCOSAHEXAENOIC acid; MARINE animal oils; BERRYTEUTHIS magister
- Publication
European Journal of Lipid Science & Technology, 2014, Vol 116, Issue 11, p1608
- ISSN
1438-7697
- Publication type
Article
- DOI
10.1002/ejlt.201400083