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- Title
A comparative proteomic study of chondral chondrocytes in osteoarthritis patients with normal chondrocytes.
- Authors
Zamani, B.; Khosravi, Gh.; Rezaei-Tavirani, M.; Hashemi-Takhonche, M.; Heidari-Keshel, S.
- Abstract
Background: Osteoarthritis (OA) is a common degenerative disease of joints and the main cause of elder patients' disability. Cartilage damage is a major problem in OA. To investigate the pathogenesis of OA, this study aimed to analyze the differential proteomics of chondrocytes in OA patients compared to normal chondrocytes. Materials and Methods: Protein extracts of cartilages prepared from 7 patients with end-stage OA, who were candidates for arthroplasty, were compared to 7 samples from normal cartilages. Proteins were analyzed by fluorescent gel electrophoresis (2D-DIGE) method and the differentially expressed proteins were identified by mass spectrometry (MS). Two samples were lost because of tissue insufficiency and the other because of mistransportation. Results: Among the 25 analyzed proteins, difference was seen for the 8 following proteins: osteonectine, cathepsin, chondroadherin, HSP, clustrine and collagen II, Activin A and carbonic Anhydrase. The levels of osteonectine, cathepsin, clustrine and collagen II were decreased in the OA samples compared to normal chondrocytes. Moreover, the levels of chondroadherin and Activin A were increased in the OA samples compared to normal chondrocytes. Conclusion: The findings demonstrate the ability of the 2D-DIGE/MS platform to identify proteins with altered expression in chondrocytes of patients with OA. The identification of these proteins may open new lines of research for the pathogenesis of OA.
- Subjects
PROTEIN analysis; CARTILAGE analysis; MASS spectrometry; OSTEOARTHRITIS; PROTEOMICS
- Publication
Feyz Journal of Kashan University of Medical Sciences, 2014, Vol 18, Issue 2, p159
- ISSN
1029-7855
- Publication type
Article