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- Title
A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice.
- Authors
Fujii, Shinya; Kazama, Tomohiko; Ito, Yukihiro; Kojima, Soichi; Toriyama, Kinya
- Abstract
Background: The pollen function of cytoplasmic male sterile (CMS) plants is often recovered by the Restorer of fertility ( Rf) gene encoded by the nuclear genome. An Rf gene of Lead rice type CMS, Rf2, encodes a small mitochondrial glycine-rich protein. RF2 is expected to function by interacting with other proteins, because RF2 has no motifs except for glycine-rich domain. Findings: To elucidate the protein that interacts with RF2, we performed yeast two-hybrid screening. We identified four genes and named RF2-interacting candidate factors ( RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria. A pull-down assay using E. coli-produced recombinant GST-tagged RF2 and His-tagged RIF2 confirmed that RF2 interacted with RIF2. RIF2 encodes ubiquitin domain-containing protein. Conclusions: These results suggest that RIF2 is a candidate factor of a fertility restoration complex of RF2.
- Subjects
MALE sterility in plants; RICE planting rites; CYTOPLASMIC granules; RECOMBINANT antibodies; STERILITY in plants
- Publication
Rice (19398425), 2014, Vol 7, Issue 1, p1
- ISSN
1939-8425
- Publication type
Article
- DOI
10.1186/s12284-014-0021-6