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- Title
Establishment of a New Murine Elastase-Induced Aneurysm Model Combined with Transplantation.
- Authors
Rowinska, Zuzanna; Gorressen, Simone; Merx, Marc W.; Koeppel, Thomas A.; Liehn, Elisa A.; Zernecke, Alma
- Abstract
Introduction: The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation. Methods: Adult male mice (n = 6–9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting. Results: Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11±0.10 mm vs. 0.75±0.03 mm; p≤0,001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts. Conclusions: We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.
- Subjects
ELASTASES; TRANSPLANTATION of organs, tissues, etc.; AORTIC aneurysms; AORTA surgery; HISTOPATHOLOGY; LABORATORY mice
- Publication
PLoS ONE, 2014, Vol 9, Issue 7, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0102648