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- Title
Responses of Rubisco and Rubisco activase in cucumber seedlings to low temperature and weak light.
- Authors
Zhen-sheng Jiang; Xiao-qi Sun; Xi-zhen Ai; Mei-ling Wang; Huan-gai Bi; Hong-tao Wang
- Abstract
Using 'Jinyou 3' cucumber seedlings as test materials, this paper studied their photo-synthetic rate (Pn) , Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase ( RCA) activities, and gene expression of Rubisco and RCA under optimal temperature and weak light (WL: 25°C/18 °C , 100 μmol ∙ m-2 ∙ s-1) , suboptimal temperature and weak light (ST+WL: 18°C/12°C , 100 μmol ∙ m-2∙s-1) , and low temperature and weak light (LT+WL: 10 °C/5°C, 100 μmol ∙ m-2 ∙ s-1). Comparing with the control (25 °C/18 °C, 400 μmol ∙ m-2 ∙ s-1) , treatments WL, ST+WL, and LT+WL all led to a remarkable decrease in leaf area and dry matter mass. At initial stage, the Pn, Rubisco activity, rbcL and rbcS expression, RCA activity, and CsRCA expression in the three treatments declined by a big margin; 5-7 days later, these parameters tended to be less changed in treatment WL, ascended slowly in treatment ST+WL, and decreased continuously in treatment LT+WL. These results suggested that the photosynthetic apparatus of test cucumber seedlings could gradually adapt to weak light or suboptimal temperature and weak light. The Rubisco and RCA activities and the gene expression of Rubisco and RCA showed the similar responses to low temperature and weak light as the Pn, suggesting that the decline in Rubisco and RCA activities and gene expression in cucumber seedlings under low temperature and weak light could be the important reason leading to the decrease of Pn.
- Subjects
CUCUMBER research; GENE expression; LIGHT absorption; LOW temperatures; SEED biotechnology; PHOTOSYNTHETIC reaction centers
- Publication
Yingyong Shengtai Xuebao, 2010, Vol 21, Issue 8, p2045
- ISSN
1001-9332
- Publication type
Article