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- Title
Quantitative Study of the Effects of Morphine on the Mouse Spleen and Inguinal Lymph Node.
- Authors
Vojdani, Zahra; Dehghani, Farzaneh; Seyedi, Fatemeh; Noorafshan, Ali; Bahaal-din Bagi, Faegheh
- Abstract
Background: Morphine, as a narcotic analgesic drug, can suppress the immune system including the spleen and lymph node during long-term administration. Quantitative studies can be an appropriate indicator for the assessment of organ disturbances. As such, this study aimed to determine the effect of chronic morphine treatment on the histological parameters of the spleen and lymph node, with the use of stereological methods. Methods: Nineteen male mice were divided into two groups, experimental and control. Addiction was induced by administration of 0.5% morphine solution in the experimental group. On day 71, after a physical dependency test, the animals were deeply anesthetized, dissected and their spleens and inguinal lymph nodes were removed. After histological preparation, the sections with a constant distance were selected and stained with hematoxilin-eosin. The total volume, red pulp, white pulp, and trabecular volume of the spleen as well as the total volume, cortex, nodule, medulla, medullary cord, and medullary sinus of the inguinal lymph node were calculated by Cavalieri's and point-counting methods. Data were analyzed with the Mann-Whitney U test. Results: The mean total volume 34.87±6.74 mm3, mean white 9.57±2.66 mm3 and red pulp volume 24.10±4.65 mm3 of the spleen in the experimental group decreased significantly [~44% (P<0.001), ~43% (P<0.003), and ~39.5% (P<0.001), respectively]. The mean cortex 4.37±1.39 mm3 and nodule volume 1.67±0.70 mm3 of the inguinal lymph node in the experimental group decreased significantly (P<0.04; ~31% and ~29.5%, respectively). In addition, the white blood cell count decreased in the experimental group 7.12±2.7 (~21%). Conclusions: Long-term use of morphine can suppress the immune system by reducing both the spleen and inguinal lymph node volume, and white blood cells.
- Subjects
MORPHINE; PHYSIOLOGICAL effects of narcotics; SPLEEN diseases; LYMPH node diseases; GROIN; PAIN management; DRUG administration; DRUG dosage; MOUSE diseases; MICE physiology; LABORATORY mice; DISEASES; DIAGNOSIS; PHYSIOLOGY
- Publication
Archives of Iranian Medicine (AIM), 2010, Vol 13, Issue 4, p294
- ISSN
1029-2977
- Publication type
Article