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- Title
Identification of haploids and diploids in maize using seedling traits and flow cytometry.
- Authors
Guedes Baleroni, Alessandra; Ré, Fabrícia; Pelozo, Andressa; Henrique Kamphorst, Samuel; Pedroza Carneiro, José Walter; Marcelo Rossi, Robson; Alberto Scapim, Carlos
- Abstract
The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature to evaluate this methodology and verify its effectiveness. Haploid and diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using the R1-Navajo marker and placed to germinated in growth chambers maintained at different temperatures. After 96 h, radicle and coleoptile lengths and the number of lateral seminal roots were determined. Cut-off points for these traits were established using Receiver Operating Characteristic curves and a new haploid/diploid classification was performed and compared to the plant phenotype (gold standard) to obtain false discovery rates and false negative rates. The seedling traits methodology successfully differentiated haploids and diploids, proving to be effective in eliminating false positives, selected by the R1-Navajo marker. The temperature of 30 °C was established as the optimal germination temperature for this study.
- Subjects
HAPLOIDY; RECEIVER operating characteristic curves; FLOW cytometry; FALSE discovery rate; SEEDLINGS
- Publication
Crop Breeding & Applied Biotechnology, 2021, Vol 21, Issue 4, p1
- ISSN
1518-7853
- Publication type
Article
- DOI
10.1590/1984-70332021v21n4a54