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- Title
Short-term in vivo inhibition of nitric oxide synthase with L-NAME influences the contractile function of single left ventricular myocytes in rats.
- Authors
Lunz, Wellington; Natali, Antônio José; Carneiro, Miguel Araújo; dos Santos Aggum Capettini, Luciano; Baldo, Marcelo Perim; de Souza, Matheus Ornelas; Quintão, Judson Fonseca; Bozi, Luiz Henrique Marchesi; Lemos, Virginia Soares; Mill, José Geraldo
- Abstract
The main purpose of this study was to investigate the effects of short-term L-NAME treatment on the contractile function of left ventricle (LV) myocytes and the expression of proteins related to Ca2+ homeostasis. Data from Wistar rats treated with L-NAME (L group, n = 20; 0.7 g/L in drinking water; 7 days) were compared with results from untreated controls (C group, n = 20). Cardiomyocytes from the L group showed increased ( p < 0.05) fractional shortening (23%) and maximum rate of shortening (20%) compared with the C group. LV from the L group also showed increased ( p < 0.05) expression of the ryanodine receptor 2 and Na+/Ca2+ exchanger proteins (76% and 83%, respectively; p < 0.05). However, the L and C groups showed similar in vivo hemodynamic parameters of cardiac function. In conclusion, short-term NOS inhibition determines an increased expression of Ca2+ regulatory proteins, which contributes to improving cardiomyocyte contractile function, preserving left ventricular function.
- Subjects
NITRIC-oxide synthases; CARDIAC contraction; HEART cells; CELL contraction; MUSCLE contraction; CALCIUM in the body; GENE expression; RYANODINE receptors; LABORATORY rats
- Publication
Canadian Journal of Physiology & Pharmacology, 2011, Vol 89, Issue 4, p305
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/y11-015