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- Title
Determination of 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) in shellfish by UPLC-QqQ/MS and evaluation of matrix effect.
- Authors
Wei, Yingxin; Cong, Peixu; Liu, Hongying; Li, Ruoshu; Liu, Ye; Yu, Yanfei; Wang, Guiping; Zheng, Hongwei; Xue, Changhu
- Abstract
3,5-Dihydroxy-4-methoxybenzyl alcohol (DHMBA) has been considered a prominent active compound in shellfish, while its confirmatory analysis method in shellfish has not been well identified. In this study, a UPLC-QqQ/MS method for rapid and accurate determination of DHMBA was developed and optimized. The matrix effect of the shellfish samples was evaluated, and the corresponding pretreatment method using 75% acetonitrile treatment was also developed. The method was evaluated under optimum conditions. The calibration curve was linear in the range of 1.0 to 750.0 ng mL−1, with correlation coefficients greater than 0.999. The limits of detection (LODs) and limits of quantification (LOQs) were calculated to be 0.38 ng mL−1 and 1.15 ng mL−1, respectively. The relative standard deviation (RSD) of intra-day and inter-day precision were 1.49 and 7.88%, respectively. The pretreatment strategies had also been optimized and by coupling to the UPLC-QqQ/MS analysis, a new strategy for the detection of DHMBA was proposed and validated with different origins and tissues of oysters and shellfishes. With the spiking concentration from 100 to 250 ng mL−1, the recovery rate was calculated in the range of 72.24–114.46%, and the RSD was lower than 12%. All these results demonstrated that the established method could be a promising technique for the monitoring of DHMBA in aquatic shellfish processing.
- Subjects
SHELLFISH; MATRIX effect; ALCOHOL; DETECTION limit; STANDARD deviations; ACETONITRILE
- Publication
Journal of Food Measurement & Characterization, 2023, Vol 17, Issue 1, p973
- ISSN
2193-4126
- Publication type
Article
- DOI
10.1007/s11694-022-01667-w